abstract：:The MRC OX-2 monoclonal antibody recognises membrane glycoproteins of Mr 41,000 in rat brain and 47,000 on thymocytes. It also reacts with follicular dendritic cells in lymphoid organs, endothelium, smooth muscle, and B-lymphocytes. Indirect immunoperoxidase staining of cryostat sections showed that OX-2 antigen was p...

journal_title：Journal of neurochemistry

authors： Webb M,Barclay AN

更新日期：1984-10-01 00:00:00

abstract：:The subcellular distribution of the ectoenzyme, 5'-nucleotidase, in cerebral cortex and cerebellum of the rat was studied both biochemically and cytochemically. The fractions were characterized biochemically by marker enzymes. The localization of 5'-nucleotidase activity was also investigated cytochemically in the mye...

journal_title：Journal of neurochemistry

authors： Heymann D,Reddington M,Kreutzberg GW

更新日期：1984-10-01 00:00:00

abstract：:The distribution of cerebellar gangliosides was studied in staggerer (sg/sg) mutant mice, where the majority of granule cells die after completing their migration across the molecular layer. In addition, the external granule cell layer in sg/sg mice persists longer than in normal mice. Moreover, in the sg/sg cerebellu...

journal_title：Journal of neurochemistry

authors： Seyfried TN,Bernard DJ,Yu RK

更新日期：1984-10-01 00:00:00

abstract：:The biosynthesis of angiotensin II-like peptide was measured in primary cultured brain cells from the fetal rat. Cells from the whole brains of 20-day gestational age Sprague-Dawley rats were dissociated by mild trypsinization and grown for 5 days in supplemented (serum-free) medium prior to experimental analysis. The...

journal_title：Journal of neurochemistry

authors： Weyhenmeyer JA,Meyer JM,Watkins JE

更新日期：1984-09-01 00:00:00

abstract：:Three fractions (one soluble and two membrane-bound) of choline acetyltransferase (ChAT) isolated from a nerve ending fraction of mouse forebrain, which have previously been reported to differ in several biochemical and physical aspects, were also found to differ in their rates of postnatal development. At 2 days of a...

journal_title：Journal of neurochemistry

authors： Benishin CG,Carroll PT

更新日期：1984-09-01 00:00:00

abstract：:We have studied the responsiveness of vascular adenylate cyclase to vasoactive intestinal peptide (VIP) and parathyroid hormone (PTH) using preparations of cerebral microvessels and arteries. Cerebral microvessels obtained from rats, guinea-pigs, cattle, and pigs all responded potently to bovine (b) PTH-(1-34), wherea...

journal_title：Journal of neurochemistry

authors： Huang M,Rorstad OP

更新日期：1984-09-01 00:00:00

abstract：:Uptake and release of alanine were measured in lumbar sympathetic chains excised from embryos of white leghorn chickens, 14-15 days old, and incubated in a modified Eagle's minimum essential medium. In the presence of [U-14C]glucose, glucose carbon accumulated in alanine in the medium at a rate that increased when unl...

journal_title：Journal of neurochemistry

authors： Larrabee MG

更新日期：1984-09-01 00:00:00

abstract：:The benzodiazepines (BZDs) chlordiazepoxide (CDE), diazepam (DZM), and flurazepam (FLM) inhibited receptor binding for thyrotropin-releasing hormone (TRH) with low micromolar potency. In contrast, numerous other categories of drugs were previously shown to be inactive. Scatchard analysis of competition data suggested ...

journal_title：Journal of neurochemistry

authors： Sharif NA,Burt DR

更新日期：1984-09-01 00:00:00

abstract：:The kinetics of the reactions of nine opioid peptides with the neutral endopeptidase ("enkephalinase") activities of human kidney, rat kidney, and rat brain have been determined. These opioid peptides can be divided into two classes, those that are good inhibitors of Leu5-enkephalin hydrolysis (Ki less than 75 microM)...

journal_title：Journal of neurochemistry

authors： Hersh LB

更新日期：1984-08-01 00:00:00

abstract：:Cathepsin D (CD) was purified to homogeneity from postmortem human cerebral cortex. Incubation of CD with human neurofilament proteins (NFPs) prepared by axonal flotation led to the rapid degradation of the 200,000, 160,000, and 70,000 NFP subunits (200K, 160K, and 70K) which had been separated by one- or two-dimensio...

journal_title：Journal of neurochemistry

authors： Nixon RA,Marotta CA

更新日期：1984-08-01 00:00:00

abstract：:MDL 72145, (E)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine hydrochloride, was designed and synthesised as a potential enzyme-activated irreversible inhibitor of monoamine oxidase (MAO). In vitro, the compound displayed time-dependent pseudo-first-order irreversible inhibitory characteristics with high selectivity for...

journal_title：Journal of neurochemistry

authors： Zreika M,McDonald IA,Bey P,Palfreyman MG

更新日期：1984-08-01 00:00:00

abstract：:Cyclic-AMP-binding proteins in membrane and soluble fractions from rat forebrain were compared; membrane fractions included smooth and rough microsomes and a plasma membrane fraction enriched in synaptic membranes. Protein fractions were treated with 8-azido-[32P]cyclic AMP and ultraviolet irradiation to covalently ta...

journal_title：Journal of neurochemistry

authors： Strocchi P,Sapirstein VS,Rubin CS,Gilbert JM

更新日期：1984-08-01 00:00:00

abstract：:The presence of 5-hydroxytryptamine was investigated in cultured and propagated cerebrovascular endothelium using immunohistochemistry and high pressure liquid chromatography. These studies demonstrate that the endothelium has the ability to take up and metabolize 5-hydroxytryptamine as well as to synthesize this amin...

journal_title：Journal of neurochemistry

authors： Maruki C,Spatz M,Ueki Y,Nagatsu I,Bembry J

更新日期：1984-08-01 00:00:00

abstract：:gamma-Aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD) activities were measured in the ovary and the Fallopian tube of rats and compared with brain values. GABA levels in the Fallopian tube were about twice as high as in the brain, while in the ovary they represented only about 5% of the amino acid conte...

journal_title：Journal of neurochemistry

authors： Apud JA,Tappaz ML,Celotti F,Negri-Cesi P,Masotto C,Racagni G

更新日期：1984-07-01 00:00:00

abstract：:Previously we found close similarities between high-affinity binding sites for [3H]cocaine and those for [3H]imipramine in the mouse cerebral cortex in regard to their association with neuronal uptake of serotonin. In the present study we investigated whether the two ligands bind to the same site. The two ligands had ...

journal_title：Journal of neurochemistry

authors： Reith ME,Allen DL,Sershen H,Lajtha A

更新日期：1984-07-01 00:00:00

abstract：:Increased arachidonic acid release occurred during activation of catecholamine secretion from cultured bovine adrenal medullary chromaffin cells. The nicotinic agonist 1,1-dimethyl-4- phenylpiperazinium (DMPP) caused an increased release of preincubated [3H]arachidonic acid over a time course which corresponded to the...

journal_title：Journal of neurochemistry

authors： Frye RA,Holz RW

更新日期：1984-07-01 00:00:00

abstract：:The effect of compounds that activate sodium channels on the number of muscarinic acetylcholine receptors in neuroblastoma NIE 115 cells has been investigated. The cells were used in electrically unexcitable ("control" cells) and excitable ("differentiated" cells) states. Although receptor assays using a single concen...

journal_title：Journal of neurochemistry

authors： Milligan G,Strange PG

更新日期：1984-07-01 00:00:00

abstract：:This paper proposes a tentative amino acid sequence of guinea pig myelin basic protein obtained by comparison of peptide fragments of the guinea pig and bovine proteins. Analyses of the tryptic peptides confirmed the known sequence differences in the NH2-terminal half of the molecule and showed that in the COOH-termin...

journal_title：Journal of neurochemistry

authors： Deibler GE,Martenson RE,Krutzsch HC,Kies MW

更新日期：1984-07-01 00:00:00

abstract：:In adult New Zealand white rabbits, the effects of food deprivation and of massive elevations of plasma uridine or thymidine concentrations on CSF and plasma nucleoside and oxypurine concentrations were studied. Nucleoside and oxypurine levels were determined by high performance liquid chromatography using unequivocal...

journal_title：Journal of neurochemistry

authors： Eells JT,Spector R,Huntoon S

更新日期：1984-06-01 00:00:00

abstract：:The effects of fatty acids, oleate and palmitate, on gamma-aminobutyric acid (GABA), aspartate, and 3,4- dihydroxyphenylethylamine (dopamine) transport and a variety of other membrane functions were studied in rat brain synaptosomes at a constant lipid-to-protein ratio. Under the conditions utilized oleate, but not pa...

journal_title：Journal of neurochemistry

authors： Troeger MB,Rafalowska U,Erecińska M

更新日期：1984-06-01 00:00:00

abstract：:In continuing studies on smooth microsomal and synaptic membranes from rat forebrain, we compared the binding properties of opiate receptors in these two discrete subcellular populations. Receptors in both preparations were saturable and stereospecific. Scatchard and Hill plots of [3H]naloxone binding to microsomes an...

journal_title：Journal of neurochemistry

authors： Roth BL,Coscia CJ

更新日期：1984-06-01 00:00:00

abstract：:An adrenomedullary protease capable of generating Met5-enkephalin from endogenous precursor(s) has been purified 1,000-fold using affinity chromatography in combination with gel filtration. This trypsin-like enzyme has an apparent molecular weight of 20,000 daltons by gel filtration. The reactivity of the enzyme towar...

journal_title：Journal of neurochemistry

authors： Lindberg I,Yang HY,Costa E

更新日期：1984-05-01 00:00:00

abstract：:The ontogeny of chick brain and heart ventricle calcium antagonist binding sites was determined, using [3H]nitrendipine ([3H]NDP), as the ligand. The binding of [3H]NDP to adult heart and brain was kinetically very similar, with the former displaying a KD of 0.28 +/- 0.02 nM and a Bmax of 138 +/- 17 fmol/mg protein, a...

journal_title：Journal of neurochemistry

authors： Marangos PJ,Sperelakis N,Patel J

更新日期：1984-05-01 00:00:00

abstract：:The distributions of glutamate decarboxylase (EC 4.1.1.15), gamma-aminobutyric acid transaminase (EC 2.6.1.19), and succinate semialdehyde dehydrogenase (EC 1.2.1.24) were determined in monkey retina. The decarboxylase was almost restricted to the inner plexiform layer. The transaminase was also highest in this layer,...

journal_title：Journal of neurochemistry

authors： Pusateri ME,Carter JG,Berger SJ,Lowry OH

更新日期：1984-05-01 00:00:00

abstract：:Microsomal fractions isolated from rat corpus striatum catalyze the oxidation of thiobenzamide to the sulfoxide. The rate of thiobenzamide sulfoxidation is 6.9 +/- 4.8 nmol(min)-1 (mg microsomal protein)-1. The reaction is inhibited by an excess of sulfur- and nitrogen-containing substrates for the microsomal flavin-c...

journal_title：Journal of neurochemistry

authors： Duffel MW,Gillespie SG

更新日期：1984-05-01 00:00:00

abstract：:Nicotinic cholinergic receptor binding sites labeled by [3H]acetylcholine were measured in the cerebral cortices, thalami, striata, and hypothalami of rats lesioned by intraventricular injection of either 6-hydroxydopamine or 5, 7-dihydroxytryptamine. In addition, [3H]acetylcholine binding sites were measured in the c...

journal_title：Journal of neurochemistry

authors： Schwartz RD,Lehmann J,Kellar KJ

更新日期：1984-05-01 00:00:00

abstract：:Addition of vasoactive intestinal peptide (VIP) to brain homogenates increased the activity of choline acetyltransferase (ChAT) but not that of acetylcholinesterase or glucose-6-phosphate dehydrogenase. Activity of ChAT was increased in the anterior hypothalamus and in the dorsal and ventral hippocampus, but not in th...

journal_title：Journal of neurochemistry

authors： Luine VN,Rostene W,Rhodes J,McEwen BS

更新日期：1984-04-01 00:00:00

abstract：:This paper describes the interaction of neurotensin with mouse neuroblastoma N1E115 cells. Neurotensin binding sites are undetectable in nondifferentiated neuroblastoma cells. They appear during cell differentiation in the presence of a low serum concentration and dimethyl sulfoxide, and reach a maximal level after 50...

journal_title：Journal of neurochemistry

authors： Poustis C,Mazella J,Kitabgi P,Vincent JP

更新日期：1984-04-01 00:00:00

abstract：:In a study employing mouse brain synaptosomes and synaptosomal sonicates, the complete metabolic machinery was found to be present for transport of arginine into synaptosomes, its conversion to ornithine, and the formation from the latter of glutamic acid, gamma-aminobutyric acid, and proline. The results show that a ...

journal_title：Journal of neurochemistry

authors： Johnson JL,Roberts E

更新日期：1984-04-01 00:00:00

abstract：:Rat glioma X mouse neuroblastoma hybrid neurotumor cells (NG108-15), synchronized by amino acid deprivation, showed a cell-cycle-dependent peak of activity of a ganglioside N-acetylgalactosaminyl transferase 14-24 h following release from the cell cycle block (S/G2 phase). Maximal expression of two typical lysosomal h...

journal_title：Journal of neurochemistry

authors： Scheideler MA,Lockney MW,Dawson G

更新日期：1984-04-01 00:00:00

abstract：:Total polyribosomes were isolated from the brains of 16-20 day C57BL/6 mice, four neurological mutants (qk/qk, shi/shi, mld/mld, and jp/Y), and four heterozygote or littermate controls (qk/+, shil/+, mld, and jp littermates) and translated in a homologous, cell-free system. No differences were observed among the nine ...

journal_title：Journal of neurochemistry

authors： Campagnoni AT,Campagnoni CW,Bourre JM,Jacque C,Baumann N

更新日期：1984-03-01 00:00:00

abstract：:Acetylcholinesterase (AChE) is found both in motor end-plate (MEP)-free and MEP-rich regions of rat or mouse muscle. We studied the developmental aspects of the localization of asymmetric 16S AChE in both regions of the sternocleidomastoid muscle, which has a well-defined zone of motor innervation. In the rat, the pro...

journal_title：Journal of neurochemistry

authors： Rieger F,Goudou D,Tran LH

更新日期：1984-03-01 00:00:00

abstract：:Knowledge of the relative contributions of mast cells and neurons to the overall pool of histamine in the brain is a prerequisite to determining the significance and role of this amine in brain function. Consequently, we analyzed the levels of brain histamine in four genotypes (+/+, W/+, Wv/+, and W/Wv) of WBB6F1 mice...

journal_title：Journal of neurochemistry

authors： Orr EL,Pace KR

更新日期：1984-03-01 00:00:00

abstract：:Preincubation of murine neuroblastoma cells (clone N1E-115) with terbium chloride resulted in a significant potentiation of carbachol-mediated increase in cyclic GMP formation. This effect was accompanied by a shift of the peak response from 30 s to 120 s and a 6-fold decrease in carbachol concentration producing half...

journal_title：Journal of neurochemistry

authors： El-Fakahany EE,Pfenning M,Richelson E

更新日期：1984-03-01 00:00:00

abstract：:A glutamate binding protein was purified from bovine brain to apparent homogeneity. The procedure used for the purification of this protein involved extraction of a crude synaptic membrane fraction with Na-cholate, followed by solubilization of the binding protein from the membranes by Triton X-100, and, finally, affi...

journal_title：Journal of neurochemistry

authors： Michaelis EK,Chittenden WL,Johnson BE,Galton N,Decedue C

更新日期：1984-02-01 00:00:00

abstract：:As a basis for attempts to define the structures of the proteins within myelin, methods have been developed for their extraction and isolation in solutions of non-denaturing detergents. With use of solutions of deoxycholate or Triton X-100, up to 90% of the protein has been extracted from bovine CNS myelin, along with...

journal_title：Journal of neurochemistry

authors： Smith R,Cook J,Dickens PA

更新日期：1984-02-01 00:00:00

abstract：:A new protein kinase modulated by S-100 (tentatively referred to as protein kinase X) was partially purified from pig brain extracts. The activity of protein kinase X, which was independent of Ca2+, was demonstrated when protamine (free base), but not protamine sulfate and other proteins (including histone), was used ...

journal_title：Journal of neurochemistry

authors： Qi DF,Turner RS,Kuo JF

更新日期：1984-02-01 00:00:00

abstract：:Benzodiazepine receptor binding was measured in cerebellar cortex of 15 patients with dominantly inherited olivopontocerebellar atrophy (OPCA). The majority of these patients had a moderate to marked Purkinje cell loss, as judged by the lowered levels of dentate nucleus gamma-aminobutyric acid (GABA), a marker of Purk...

journal_title：Journal of neurochemistry

authors： Kish SJ,Perry TL,Hornykiewicz O

更新日期：1984-02-01 00:00:00

abstract：:The turnover rate of gamma-aminobutyric acid (GABA) in the rat striatum was estimated by measuring its accumulation after inhibition of GABA-transaminase (GABA-T) with gabaculine. Intrastriatal injections of 100 micrograms gabaculine induced a rapid and complete inhibition of GABA-T. GABA accumulation was linear with ...

journal_title：Journal of neurochemistry

authors： Giorgi O,Meek JL

更新日期：1984-01-01 00:00:00

abstract：:This study investigates the functioning of synaptosomal ouabain-sensitive Na+ -K+ -ATPase in cold-induced edema. During vasogenic brain edema development, the enzyme affinities for Na+ and K+ are progressively decreased paralleling the increase in the tissue water content, whereas maximal velocity of the reaction is n...

journal_title：Journal of neurochemistry

authors： Averet N,Rigoulet M,Cohadon F

更新日期：1984-01-01 00:00:00